Bioinformatics services- 16S metagenomics

16S metagenomics involves sequencing the 16S rRNA gene to profile microbial communities. This method is widely used for identifying and quantifying bacteria and archaea in environmental, clinical, or biological samples.

Pipeline Overview

1. Demultiplexing: Sorting sequencing reads based on sample barcodes.
2. Quality Control (QC): Evaluating raw data for quality and filtering low-quality sequences.
3. Primer Removal: Removing primers from the 16S rRNA gene sequences.
4. OTU Clustering: Grouping similar sequences into operational taxonomic units (OTUs) or amplicon sequence variants (ASVs) using tools like DADA2.
5. Taxonomic Assignment: Assigning taxonomic classifications to OTUs using reference databases like SILVA.
6. Visualization: Visualizing microbial diversity with QIIME2.

Expected Result Output

1. Main Results File: A .csv file with taxonomic abundances at different levels (e.g., genus, species).
2. Additional Results:
   • Bar Chart: Showing microbial abundance at different taxonomic levels.
   • Alpha/Beta Diversity Plots: To assess diversity within and between samples.