User-prepared library sequencing

The ATGC provides comprehensive sequencing services for user-prepared libraries utilizing our advanced Illumina sequencers: NextSeq 2000 and MiSeq. This service is designed for researchers who wish to prepare their own sequencing libraries and submit them for sequencing at our facility. Our team ensures the highest quality sequencing outcomes, while the responsibility for library preparation and compatibility with Illumina sequencing standards lies with the user.

Key user responsibilities:

• Library compatibility: Users must ensure their libraries are compatible with Illumina sequencing platforms, specifically in terms of sequencing primers used in custom library preparations.
  For more information please visit:
  Nextseq2000
  Miseq 
  Custom library preparations may require special considerations for primer sequences. Please contact us on this matter.
• PhiX control: The inclusion of PhiX Control v3 Library is essential to ensure accurate base calling, especially when libraries have low base diversity. By default, we add 2% PhiX to all sequencing runs. However, it is the user’s responsibility to determine if a higher percentage of PhiX is necessary for optimal results. For additional info

 

The following flow cells are available for the NextSeq 2000 sequencer:

Flow cell	Max read length (single read)	Max read length (paired-end)	Number of expected reads (millions)
P1 100 cycles	100 bps	2*50 bp	100 M
P1 300 cycles	300 bp	2*150 bp
P1 600 cycles	-	2*300 bp
P2 100 cycles	100 bp	2*50 bp	400 M
P2 200 cycles	150 bp	2*100 bp
P2 300 cycles	-	2*150 bp
P2 600 cycles	-	2*300 bp	300 M
P3 100 cycles	100 bps	2*50 bp	1200 M
P3 200 cycles	150 bp	2*100 bp
P3 300 cycles	-	2*150 bp
P4 50 cycles	50 bp		1800M
P4 100 cycles	100 bps	2*50 bp
P4 200 cycles	150 bp	2*100 bp
P4 300 cycles	-	2*150 bp

 

The following flow cells are available for the Miseq sequencer:

Flow cell	Max read length (single read)	Max read length (paired-end)	Number of expected reads (millions)
Nano 300 cycles	300 bp	2*150 bp	1 M
Nano 500 cycles	-	2*250 bp
Micro 300 cycles	300 bp	2*150 bp	4 M
300 V2	300 bp	2*150 bp	12 M
500 V2	-	2*250 bp
150 V3	150 bp	2*75 bp	20 M
600 V3	300 bp	2*300 bp

 

Before submitting samples for our sequencing service, please read our samples delivery instructions, fill and send us the electronic sample sheet. Make sure your barcode sequences are correct and in the Forward orientation.

The sequencing data is sent via Illumina Basespace cloud. Please open an account and indicate the email address associated with your account in the sample submission form. Instructions on how to open an account and manage your data in the cloud.