Single Cell Transcriptomics

-ATGC offers a range of single-cell and single-nuclei workflows for profiling heterogeneous cell populations, enabling the detection of cell-type- and cell-state-specific signals that may be masked by bulk measurements. Our services include single-cell transcriptomics and multi-omics applications for fresh, frozen, and FFPE samples.

Using 10x Genomics technology, ATGC offers a range of single-cell and single-nuclei applications, including Single Cell Gene Expression, Single Cell Immune Profiling, Epi ATAC, and Epi Multiome ATAC + Gene Expression. These workflows enable high-resolution profiling of cellular composition, gene expression, immune repertoires, chromatin accessibility, and integrated epigenomic and transcriptomic profiles.

ATGC also offers Illumina Single Cell 3′ RNA sequencing, a flexible alternative for single-cell gene expression profiling. The microfluidics-free PIPseq workflow supports a broad range of cell populations and sample types, including fragile and rare cells, and is scalable from hundreds to hundreds of thousands of cells.

 

Before preparing and delivering your samples, please review the sample submission instructions for your selected platform: 10x Genomics Single-Cell,  Illumina Single Cell 3′ RNA Prep. Please complete the single-cell submission form after reviewing the relevant instructions.

 

10x Genomics Single-Cell User Protocols: 

• Cell prep
• Cell thawing protocols
• Nuclei prep
• Nuclei Isolation using 10x kit
• Cell counting
• Dead cell removal
• Cell fixation GEM-X fresh
• Tissue fixation dissociation GEM-X Flex
• Cell and nuclei fixation GEM-X Flex
• Cells from FFPE tissue sections
• Nuclei Isolation for 10x Multiome - ATAC + Gene Expression Sequencing
• Nuclei Isolation for 10x Multiome - ATAC + Gene Expression Sequencing - from Complex Tissues

Illumina Single-Cell 3′ RNA Prep Users Protocols

• Illumina Single-Cell- Sample Preparation for Fresh/Frozen Cells and Nuclei
• Illumina Single Cell- DSP-Methanol Fixation for Cells
• Illumina Single Cell- DSP-Methanol Fixation for Nuclei