Single Cell Transcriptomics
Single Cell Transcriptomics
ATGC offers 10x Genomics single cell transcriptomics to perform profiling of heterogeneous cell populations from hundreds of thousands of single cells to ensure biologically relevant signals are not masked by bulk average measurements.
The optimal input cell/nuclei concentration depends upon the desired cell/nuclei recovery target, see below:
| Optimal input cell concentration | Cell recovery target |
| 700 - 1,200 cells/μl | 500 - 10,000 cells |
| 1,300 - 1,600 cells/μl | 10,000 - 20,000 cells |
| Optimal input nuclei concentration | Nuclei recovery target |
| 500 nuclei/μl | 160 - 400 nuclei |
| 1,000 nuclei /μl | 320 - 810 nuclei |
| 2,000 nuclei /μl | 650 - 1,610 nuclei |
| 3,000 nuclei /μl | 970 - 2,420 nuclei |
| 4,000 nuclei /μl | 1,290 - 3,230 nuclei |
| 5,000 nuclei /μl | 1,610 - 4,030 nuclei |
| 6,000 nuclei /μl | 1,940 - 4,840 nuclei |
| 7,000 nuclei /μl | 2,260 - 5,650 nuclei |
| 8,000 nuclei /μl | 2,580 - 6,450 nuclei |
| 9,000 nuclei /μl | 2,900 - 7,260 nuclei |
| 10,000 nuclei /μl | 3,230 - 8,060 nuclei |
In addition to scRNA-seq, the ATGC also offers additional 10x Genomics applications in fresh, frozen and FFPE tissue, such as Single Cell Immune Profiling, Epi ATAC and Epi Multiome ATAC + Gene Expression.
For details on sample delivery please read the sample delivery instructions and use the links below for the relevant protocol. Furthermore, please fill out and send us the electronic sample sheet
For additional information, please contact:
Liat Linde, Head of Azrieli Technion Genomics Center
Rappaport Building: 073-3785452
Emerson Building: 073-3785168