Sanger sequencing
Sanger sequencing utilizing the selective incorporation of chain-terminating dideoxynucleotides to accurately determine nucleotide sequences. The ATGC offers Sanger service capable of producing long contiguous DNA sequences of approximately 900 nucleotides. To ensure the reliability of your data, we perform rigorous quality control checks on all reactions before delivering the results.
Available Primers for Plasmids:
| Primer name | Primer sequence |
| T7 | TAATACGACTCACTATAGGG |
| T7-short | AATACGACTCACTATAG |
| T7-Ter | GCTAGTTATTGCTCAGCGG |
| T7 prom | TAATACGACTCACTATAGGGAGA |
| T3 | ATTAACCCTCACTAAAGGGA |
| SP6 | TATTTAGGTGACACTATAG |
| SP6 Universal | TACGATTTAGGTGACACTATAG |
| (-20) M13 F | TGTAAAACGACGGCCAGT |
| (-20) M13 R | AACAGCTATGACCATGATTA |
| (-40) M13 F | GTTTTCCCAGTGACGCA |
| PGL F | CTAGCAAAATAGGCTGTCCC |
| PGL R | GAAATACAAAAACCGCAGAAGG |
| MV PRO | ATTGACGCAAATGGGCGGTA |
| PGEX3 R | CCGGGAGCTGCATGTGTCAGAGG |
For efficient data analysis, we recommend downloading the free Chromas software from Technelysium's website. After installation, you can import your ".ab1" files into Chromas to view and analyze the electropherograms. For results analysis troubleshooting, please see Thermo-Fisher Troubleshooting Manual.
For CRISPR-Cas9 genome editing analysis, Thermo Fisher offers the SeqScreener Gene Edit Confirmation App (SGC) on Thermo Fisher™ Connect. Registration (free) is required.
Before submitting your Sanger order, please review our Sanger Sequencing sample requirements. For placing Sanger sequencing service orders, please visit our Booking webpage and our Sanger Sequencing Order Management in BookitLab manual.
For additional information, please contact:
Liat Linde, Head
Rappaport Building: 073-3785452
Emerson Building: 073-3785168
Nitsan Fourier, Lab manager