Real-Time qPCR

Quantitative PCR (qPCR), also called real-time PCR, is an assay that can be used to precisely quantitate a nucleic acid sequence of interest. One of the most common applications of qPCR is gene expression analysis, for example, an increase or decrease in the expression of several target genes in response to different conditions or treatments. qPCR is a targeted approach for relative gene expression analysis in a small-scale, as opposed to RNA-seq. It can also be applied to the analysis of gDNA targets, applications such as genotyping/SNP determination, methylation analysis, screening transgenic sequences, or monitoring of insertions and deletions.

At the ATGC, we provide comprehensive qPCR services to meet diverse research needs. Researchers can choose between:

• Independent instrument access: For researchers preferring hands-on work, we offer access to our qPCR instruments, allowing you to run your own experiments with full flexibility and control. For more information regarding independent use, please visit our QuantStudio 1 and 3 Real-Time qPCR webpage.
• Full-service qPCR: Our team utilizes validated TaqMan reagents to deliver accurate and reliable results, ensuring high-quality data for your projects. Please contact us for additional info.