Long-read sequencing

At the ATGC, we utilize the Oxford Nanopore MinION sequencer to provide comprehensive long-read sequencing services, leveraging advanced nanopore technology for both DNA and RNA analysis.

Our DNA sequencing services offer ultra-long read lengths (up to hundreds of kilobases) and enable the detection of native DNA methylation. This approach is particularly beneficial for applications such as long amplicon sequencing (greater than 500 base pairs), de novo sequencing of small genomes, and genomic editing analysis. These capabilities are applicable across various research fields, including microbiology, environmental studies, microbiome analysis, human genetics, cancer research, and plant and animal studies.

In addition to DNA sequencing, we offer RNA sequencing services on the MinION sequencer. This includes cDNA sequencing, where RNA is converted into complementary DNA, and direct RNA sequencing, which reads native RNA molecules without prior conversion. Notably, direct RNA sequencing allows for the detection of RNA modifications, providing deeper insights into transcriptome complexity. These methods enable comprehensive analysis of full-length RNA transcripts, offering valuable information on gene expression and transcript variants.

Before submitting samples for Nanopore sequencing, please review our sample delivery instructions and send us your sample submission form.