DNA Methylation

DNA methylation is an epigenetic modification involving the addition of a methyl group to cytosine residues, which can influence gene expression and cellular function.

At the ATGC, DNA methylation is analyzed using Reduced Representation Bisulfite Sequencing (RRBS), a bisulfite sequencing-based approach that targets a subset of the genome enriched for CpG-dense regions, including gene promoters, enhancers, and other regulatory regions. By selectively enriching these regions, RRBS enables high-coverage methylation profiling while requiring substantially less sequencing than whole-genome bisulfite sequencing. This makes RRBS well suited for studying DNA methylation across diverse biological samples and experimental designs.

Before submitting samples for RRBS, please read our sample delivery instructions, and fill out and send us the sample submission form.

The sequencing data is sent via Illumina Basespace cloud. Please open an account and indicate the email address associated with your account in the sample submission form. Instructions on how to open an account and manage your data in the cloud.