DNA Methylation

DNA methylation is a biochemical process involving the addition of a methyl group to the cytosine that can alter gene expression.

At the ATGC we offer two methodologies to analyze DNA methylation:

• Reduced Representation Bisulfite Sequencing (RRBS), a bisulfite sequencing-based technique that focuses on a reduced fraction of the genome, specifically enriching for CpG-dense regions, such as gene promoters, enhancers, and regulatory regions. By selectively capturing these regions, RRBS allows for deep and high-coverage sequencing while minimizing sequencing costs compared to whole-genome bisulfite sequencing. This makes it an ideal choice for large-scale studies of DNA methylation in diverse biological contexts.
• Illumina Infinium MethylationEPIC beadchips V2, a high-throughput microarray designed to measure DNA methylation across over 935K CpG sites in the human genome.
• If you are interested in other Illumina Infinium arrays (methylation of SNP), please contact us to discuss your specific requirements.

Before submitting samples for RRBS, please read our samples delivery instructions, fill and send us the electronic sample sheet.

For Illumina Infinium Methylation project, please review our  samples delivery instructions and send us your sample submission form.

The sequencing data is sent via Illumina Basespace cloud. Please open an account and indicate the email address associated with your account in the sample submission form. Instructions on how to open an account and manage your data in the cloud.