CEL-Seq2

CEL-Seq is a 3’-RNA sequencing technique in which only the 3’-UTR of the mRNA transcript is sequenced. This is enough to identify and quantify the gene’s expression. The protocol is based on polyA enrichment, but it is compatible with a wide range of mRNA quality and quantity.

In this protocol, samples are barcoded and pooled before linear amplification of mRNA via In-Vitro Transcription (IVT). During sample preparation, each mRNA transcript is tagged with a Unique Molecular Identifier (UMI), which is used during the bioinformatics analysis to collapse reads originating from the same transcript.

Before submitting samples for CEL-Seq2, please review our samples delivery instructions and send us your sample submission form.